HPRT antibody (70R-12561)
Affinity purified Rabbit polyclonal HPRT antibody
Overview
Overview
| Synonyms | Polyclonal HPRT antibody, Anti-HPRT antibody, hypoxanthine-guanine phosphoribosyltransferase antibody, HGPRT antibody, HPRT1 antibody, HPRT antibody, HGPRTase antibody, hypoxanthine phosphoribosyltransferase 1 antibody |
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| Cross Reactivity | Human |
| Applications | ICC, IF, IHC-P, WB |
| Immunogen | HPRT antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 218 of HPRT |
Images
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | HPRT antibody was purified by antigen-affinity chromatography |
| Molecular Weight | 25 kDa |
| Form & Buffer | Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative. |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:1000, WB: 1:500-1:3000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate |
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