HPRT antibody (70R-12561)

Affinity purified Rabbit polyclonal HPRT antibody

Synonyms Polyclonal HPRT antibody, Anti-HPRT antibody, hypoxanthine-guanine phosphoribosyltransferase antibody, HGPRT antibody, HPRT1 antibody, HPRT antibody, HGPRTase antibody, hypoxanthine phosphoribosyltransferase 1 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen HPRT antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 218 of HPRT

Images

Immunofluorescent staining using HPRT antibody (70R-12561)

Immunofluorescence analysis of methanol-fixed A549, using HPRT antibody at 1:500 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification HPRT antibody was purified by antigen-affinity chromatography
Molecular Weight 25 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using HPRT antibody (70R-12561)</font></div>     | Immunofluorescence analysis of methanol-fixed A549, using HPRT antibody at 1:500 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using HPRT antibody (70R-12561)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: IMR32) using HPRT antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using HPRT antibody (70R-12561)</font></div>     | Immunohistochemical staining of paraffin-embedded AdCA using HPRT antibody at a dilution of 1:500

Availability: In stock

Price: €323.11
Size: 100 ul
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