HSP27 antibody (20R-1447)

Rabbit polyclonal HSP27 antibody

Synonyms Polyclonal HSP27 antibody, Anti-HSP27 antibody, HSP-27 antibody, HSPB2 antibody, Heat Shock 27Kda Protein 2 antibody, HSP27 antibody, Hs.78846 antibody, LOH11CR1K antibody, MGC133245 antibody, MKBP antibody, HSP27, HSP 27,HSP-27,HSP 27 antibody, HSP-27 antibody
Cross Reactivity Human/Monkey
Applications ELISA, ICC, IF, IP, WB
Immunogen HSP27 antibody was raised in Rabbit using recombinant human HSP27 as the immunogen.

Specifications

Host Rabbit
Isotype IgG
Form & Buffer Supplied in PBS buffer, pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations ELISA: 1 ug/ml, IP: 5-10 ug/ml, ICC: 10-15 ug/ml, WB: 1-2 ug/ml

Storage & Safety

Storage Store at -20 deg C. For long-term storage, aliquot and freeze at -70 deg C. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Human HSP27, mouse HSP25 and alpha/beta crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. HSP27 is believed to exist mainly as oligomers of as many as 8 40 HSP27 protein monomers in cells and data suggests that the large oligomers of HSP27 have a chaperone like activity by serving as a site where unfolding proteins may bind until ATP and HSP70 dependent refolding can occur. HSP27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing HSP27. Data from studies using wild type HSP27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of HSP27. Recent findings indicate that HSP27 is also a negative regulator of Cytochrome C dependent activation of procaspase 3.

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Availability: In stock

Price: €519.43
Size: 100 ug
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