Hsp27 protein (30R-1074)
Purified recombinant Human Hsp27 protein
|Synonyms||Growth-related 25 kDa protein protein, CMT2F protein, Hsp27, Heat shock 27 kDa protein protein, Heat Shock Protein 27 protein, Hsp 28 protein, HspB1 protein, HSP 27 protein, Hsp28 protein, P25 protein, Stress responsive protein 27., DKFZp586P1322 protein, Heat shock 27kDa protein 1 protein, Estrogen regulated 24 kDa protein protein, HS.76067 protein, Hsp27 protein, Heat shock 27 kDa protein 28 kDa heat shock protein protein, Hsp-27 protein, Hsp25 protein, Hsp 27, at shock 25kDa protein 1 protein, Heat shock protein beta-1 protein, Hsp-27, Hspb1 protein, Hsp B1 protein, Hsp27 protein, Heat shock 28kDa protein 1 protein, Heat shock protein beta 1 protein, HspB1 protein, HSP25 protein, SRP27 protein, Heat Shock Protein27 protein, Hsp 27 protein|
Coomassie Blue stained SDS-PAGE of Hsp27 protein (30R-1074)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK|
|Grade & Purity||> 95% pure|
|Molecular Weight||22.7 kDa (205aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Hepes, pH 7.5, containing 1mM DTT, and 100mM KCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant human Hsp27 was overexpressed in E. coli and purified by conventional chromatography.|
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