HSP60 antibody (10R-1076)

Mouse monoclonal HSP60 antibody

Synonyms Monoclonal HSP60 antibody, Anti-HSP60 antibody, CPN 60 antibody, Heat shock 60kD protein 1 (chaperonin) antibody, wu:fa04a05 antibody, wu:fb22d10 antibody, Hsp 60 antibody, HSP -60, GroEL antibody, HuCHA60 antibody, HSPD1 antibody, HuCHA60 antibody, Heat shock 60kDa protein 1 60 kDa chaperonin antibody, Heat shock 60kD protein 1 chaperonin antibody, HSP 60 antibody, Heat shock protein 65 antibody, coli antibody, CPN60 antibody, sb:cb144 antibody, cb863 antibody, wu:fi27b05. antibody, GroEL Homolog antibody, Heat shock protein 1 (chaperonin) antibody, E antibody, Chaperonin antibody, fb22d10 antibody, 60kDa antibody, Short heat shock protein 60 Hsp60s1 antibody, Heat shock 60kDa protein 1 antibody, fa04a05 antibody, SPG 13 antibody, HSP60, Chaperonin 60 antibody, HSP 65 antibody, Heat Shock Protein 60 antibody, Spastic paraplegia 13 (autosomal dominant) antibody, id:ibd2197 antibody, HSP 60, wu:fi04a12 antibody, Spastic paraplegia 13 Mitochondrial matrix protein P1 antibody, P60 lymphocyte protein antibody, HSP65 antibody, SPG13 antibody, GroEL antibody, fi27b05 antibody, 60 kDa heat shock protein mitochondrial antibody, HSPD 1 antibody, HSP -60 antibody, heat shock 60kDa protein 1 (chaperonin) antibody, Chaperonin antibody, homolog of antibody, HSP60 antibody, 60-KD antibody, HLD4 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen HSP60 antibody was raised in mouse using recombinant human Hsp60 (1-573aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of HSP60 antibody (10R-1076)

Cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Hsp60 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 2E4
Isotype IgG1 kappa
Source Anti-human Hsp60 antibody, clone 2E4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp60 protein.
Method of Purification HSP60 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:50-1:100, WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding.

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Images

  • Western Blot analysis of HSP60 antibody (10R-1076) | Cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Hsp60 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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