Hsp90 antibody (10R-1047)

Mouse monoclonal Hsp90 antibody

Synonyms Monoclonal Hsp90 antibody, Anti-Hsp90 antibody, HSPCB antibody, Hsp90, HSPCA antibody, HSPCAL3 antibody, Heat shock 90kDa protein 1 alpha antibody, Renal carcinoma antigen NY-REN-38 antibody, Hsp 90, HSPC1 antibody, Hsp -90 antibody, HSP86 antibody, HSP90N antibody, Hsp -90, HSP90AA1 antibody, HSP90A antibody, Lipopolysaccharide associated protein2 antibody, LAP2 antibody, HSP84 antibody, FLJ26984 antibody, Heat shock protein 90-alpha Renal carcinoma antigen NY REN 38 antibody, class A member 2 antibody, FLJ31884 antibody, HSP89A antibody, D6S182 antibody, Hsp89 antibody, HSP 86 antibody, Hsp 90 antibody, Hsp90 antibody, LPS associated protein 2 antibody, HSP90alpha antibody, HSPN antibody, heat shock protein 90kDa alpha (cytosolic) antibody, hsp90 antibody, NY REN 38 antigen antibody, Heat shock protein HSP 90-alpha antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen Hsp90 antibody was raised in mouse using recombinant human Hsp90 (1-732aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of Hsp90 antibody (10R-1047)

Cell lysates of HeLa(each 20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp90 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 4F10
Isotype IgG2b kappa
Source Anti-human Hsp90 antibody, clone 4F10, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp90 protein.
Method of Purification Hsp90 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:100-1:200, WB: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Hsp90 is a human heat shock protein. In response to adverse change in their environment all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under non-stress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin.

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Images

  • Western Blot analysis of Hsp90 antibody (10R-1047) | Cell lysates of HeLa(each 20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp90 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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