HspA6 antibody (70R-12964)

Affinity purified Rabbit polyclonal HspA6 antibody

Synonyms Polyclonal HspA6 antibody, Anti-HspA6 antibody, heat shock 70 kDa protein B' antibody, HspA6, HspA-6 antibody, heat shock 70kDa protein 6 (Hsp70B') antibody, HspA 6 antibody, HspA6 antibody, heat shock 70kD protein 6 (Hsp70B') antibody, HspA 6, HspA-6, heat shock 70 kDa protein 6 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen HspA6 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 377 and 628 of HspA6

Images

Immunofluorescent staining using HspA6 antibody (70R-12964)

Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSPA6 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification HspA6 antibody was purified by antigen-affinity chromatography
Molecular Weight 71 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using HspA6 antibody (70R-12964)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A431, using HSPA6 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using HspA6 antibody (70R-12964)</font></div>     | Immunohistochemical staining of paraffin-embedded SG xenograft using HspA6 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using HspA6 antibody (70R-12964)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: H1299) using a 7.5 % SDS PAGE gel and HspA6 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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