Human IgG Fab'2 (31R-1038)
Purified Human IgG Fab'2 for use as a control or blocking reagent
Overview
Overview
| Synonyms | Human Immunoglobulin G Fab'2 |
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| Species | Human |
| Applications | User optimized |
Specifications
| Source | Normal Human Serum/Plasma was obtained from healthy donors of US origin. |
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| Grade & Purity | > 95% pure |
| Form & Buffer | Clear, colorless liquid, 0.2 um filtered, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride and 0.05% (w/v) Sodium Azide. Protease/IgG free. |
| Concentration | >4.5 mg/ml |
Usage & Assay Information
| Usage Recommendations | The optimal working dilution should be determined by the end user |
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Storage & Safety
| Storage | Store at 2-8 deg C. |
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| Biohazard Information | Donor serum was tested by FDA approved methods and found negative for antibodies to HIV1/HIVII and HCV. Donor serum was tested by FDA approved methods and found negative for HBsAg, HIV-1 RNA, HCV RNA and Syphilis. |
General Information
| Biological Significance | Fab'2 fragments of antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact some of the hinge region. Fab'2 fragments have two antigen-binding Fab portions linked together by disulfide bonds, and therefore are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of antibodies to live cells with Fc receptors or to Protein A or Protein G. |
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