Recognizes endogenous levels of Caspase 1 p10 protein
Cross Reactivity
Mouse, Rat
Applications
ICC, IF, WB
Immunogen
Caspase 1 p10 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Caspase 1 as the immunogen
Western blot analysis using ICE antibody (70R-49450)
Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.
ICE antibody
Western blot analysis using ICE antibody (70R-49450)
Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.
Immunofluorescent analysis using ICE antibody (70R-49450)
Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
ICE antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
Caspase 1 p10 is a thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.