ICE antibody (70R-49450)

Purified Polyclonal ICE antibody

Synonyms Polyclonal ICE antibody, Anti-ICE antibody, IL1BC antibody, IL1BCE antibody, Caspase-1 antibody, CASP-1 antibody, Interleukin-1 beta convertase antibody, IL-1BC antibody, Interleukin-1 beta-converting enzyme antibody, IL-1 beta-converting enzyme antibody, p45 antibody
Specificity Recognizes endogenous levels of Caspase 1 p10 protein
Cross Reactivity Mouse, Rat
Applications ICC, IF, WB
Immunogen Caspase 1 p10 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Caspase 1 as the immunogen

Images

Western blot analysis using ICE antibody (70R-49450)

Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ICE antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Caspase 1 p10 is a thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.

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Images

  • 

Western blot analysis using ICE antibody (70R-49450)



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Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.
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Immunofluorescent analysis using ICE antibody (70R-49450)



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Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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