IdhA protein (80R-4383)
Purified Recombinant IdhA protein (His tagged)
Overview
Overview
| Synonyms | fermentative D-lactate dehydrogenase protein, NAD-dependent protein, ECK1377 protein, hslF protein, hslI protein, htpH protein, JW1375 protein |
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| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Specifications
| Residues | 1-329 aa: MGSSHHHHHH SSGLVPRGSH MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 39.1 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 100mM NaCl |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Bioactivity | Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C. |
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Storage & Safety
| Storage | Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles. |
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General Information
| Biological Significance | Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. |
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