IdhA protein (80R-4383)

Purified Recombinant IdhA protein (His tagged)

Synonyms fermentative D-lactate dehydrogenase protein, NAD-dependent protein, ECK1377 protein, hslF protein, hslI protein, htpH protein, JW1375 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis using IdhA protein (His tag) (80R-4383)

Specifications

Residues 1-329 aa: MGSSHHHHHH SSGLVPRGSH MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 39.1 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 100mM NaCl
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Storage & Safety

Storage Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c.

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Images

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SDS-PAGE analysis using IdhA protein (His tag) (80R-4383)



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Availability: In stock

Price: €102.25
Size: 20 ug
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