IdhA protein (His tag) (80R-2377)
Purified recombinant E.coli IdhA protein (His tag)
Overview
Overview
| Synonyms | JW1375 protein, hslI protein, NAD-dependent protein, htpH protein, hslF protein, ECK1377 protein, fermentative D-lactate dehydrogenase protein |
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| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of IdhA protein (His tag) (80R-2377)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | MGSSHHHHHH SSGLVPRGSH MGSHMKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP LTPENYHLLN EAAFEQMKNG VMIVNTSRGA LIDSQAAIEA LKNQKIGSLG MDVYENERDL FFEDKSNDVI QDDVFRRLSA CHNVLFTGHQ AFLTAEALTS ISQTTLQNLS NLEKGETCPN ELV |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 39.1 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 100 mM NaCl |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E.coli IdhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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