idnk protein (80R-4386)

Purified Recombinant idnk protein (His tagged)

Synonyms D-gluconate kinase protein, thermosensitive protein, ECK4261 protein, gntV protein, JW4225 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis using idnk protein (His tag) (80R-4386)

Specifications

Residues 1-187 aa: MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 23.4 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 80 units/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.

Storage & Safety

Storage Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype.

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Images

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SDS-PAGE analysis using idnk protein (His tag) (80R-4386)



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Availability: In stock

Price: €102.25
Size: 20 ug
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