idnk protein (His tag) (80R-3968)
Recombinant E.coli idnk protein
Overview
Overview
| Synonyms | Recombinant idnk protein D-gluconate kinase protein, thermosensitive protein, ECK4261 protein, gntV protein, JW4225 protein |
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| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie blue stained SDS-PAGE showing analysis of idnk protein (His tag) (80R-3968)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-187 aa; MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 23.4 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as liquid in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1 mM DTT |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for 1-2 weeks. For long term storage, aliquot and store at -20 to -70 deg C. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Recombinant E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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