IFN gamma antibody (10R-6643)
Rat monoclonal IFN gamma antibody
Overview
Overview
| Synonyms | Monoclonal IFN gamma antibody, Anti-IFN gamma antibody, Interferon-gamma antibody |
|---|---|
| Specificity | Mouse |
| Applications | Functional Assay, IP, WB |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
ELISA Assay using IFN gamma antibody (10R-6643)
Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in Mouse IFN gamma ELISPOT assay. Control well is medium alone.
Specifications
| Host | Rat |
|---|---|
| Clone | AN-18 |
| Isotype | IgG1 kappa |
| Method of Purification | IFN gamma antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The AN-18 antibody reacts with mouse interferon-gamma, a 15-20 kDa cytokine secreted by Th1 cells. |
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