IFN gamma protein (30R-2267)
Purified recombinant Human IFN gamma protein
Overview
Overview
| Synonyms | Interferon-gamma protein |
|---|---|
| Specificity | Human |
| Species | Human |
| Protein Type | Recombinant |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Molecular Weight | 16 kDa |
| Form & Buffer | Supplied as a sterile liquid with phosphate buffered saline, pH7.2, 0.22 um filtered. |
| Concentration | 5 ul (0.125 ug)/test |
Usage & Assay Information
| Usage Recommendations | FC: = 0.5 ug/test |
|---|---|
| Bioactivity | The ED50 of this protein, as measured by EMC virus protection assay using A549 cells, is less than or equal to 175 pg/ml. This corresponds to a specific activity ofgreater than or equal to 5.7 x 10e6 Units/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Human IFN gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN gamma does not affect its biological activity. |
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