IGF I protein (30R-2350)
Purified recombinant Human IGF1 protein
Overview
Overview
| Synonyms | Insulin-like Growth Factor-I protein, IGF-IA protein, Somatamedin C protein, IGF1 protein, IGF 1 protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | Functional Assay |
Specifications
| Residues | Human IGF1 with modified N-terminus with a R3 chain. |
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| Expression System | E.coli |
| Grade & Purity | > 97% pure |
| Method of Purification | IGF I protein was purified using a a proprietary chromatographic technique. |
| Molecular Weight | 9.11 kDa |
| Form & Buffer | Supplied in lyophilized form with no additives. Reconstitute in distilled water or 10 mM AcOH to 1 mg/ml. |
Usage & Assay Information
| Bioactivity | Human IGF1 with added R3 chain offers higher potency than regular human IGF1 in bioactivity assay. The ED50 as determined by a cell proliferation assay using NIH3T3 cells is typically 1-6 ng/ml. |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | <0.1 ng/ug |
General Information
| Biological Significance | IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Highly potent form of endocrine hormone IGF1. |
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