IGF1 protein (30R-AI046)

Purified recombinant Human IGF1 protein

Synonyms IGF-IA protein, IGF1 protein, Somatomedin C protein, MGF protein, IGF-1 protein, IGF-1, IGF-I protein, Mechano growth factor protein, IGF 1, Insulin-Like Growth Factor 1 protein, IGFI protein, IGF1, IGF 1 protein
Species Human
Protein Type Recombinant
Applications Functional Assay
Assay Information Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.

Specifications

Residues Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7655 Dalton. IGF-I is purified by proprietary chromatographic techniques
Expression System E.coli
Grade & Purity > 98% pure
Form & Buffer Supplied as a lyophilized powder. Reconstitute in distiled water to a concentration of 0.1-1.0 mg/ml.

Usage & Assay Information

Bioactivity The ED50, calculated by the dose-dependant proliferation of murine BALB\\C 3T3 cells (measured by 3H-thymidine uptake) is

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).

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Availability: In stock

Price: €305.78
Size: 1 mg
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