IGF1 protein (30R-AI046)
Purified recombinant Human IGF1 protein
Overview
Overview
| Synonyms | IGF-IA protein, IGF1 protein, Somatomedin C protein, MGF protein, IGF-1 protein, IGF-1, IGF-I protein, Mechano growth factor protein, IGF 1, Insulin-Like Growth Factor 1 protein, IGFI protein, IGF1, IGF 1 protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | Functional Assay |
| Assay Information | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard. |
Specifications
| Residues | Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7655 Dalton. IGF-I is purified by proprietary chromatographic techniques |
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| Expression System | E.coli |
| Grade & Purity | > 98% pure |
| Form & Buffer | Supplied as a lyophilized powder. Reconstitute in distiled water to a concentration of 0.1-1.0 mg/ml. Please Note: Buffer may change with new Lots. |
Usage & Assay Information
| Bioactivity | The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003). |
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