Monoclonal IgM antibody, Anti-IgM antibody, Anti IgM antibody, Immunoglobulin M antibody, IgM antibody, Ig M antibody, Immunoglobulin M antibody, Ig M antibody
Specificity
Mouse
Applications
FC, Functional Assay, IHC, IP
Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Store at 2-8 deg C. Do not freeze. Protect from light.
General Information
Biological Significance
The HIS40 monoclonal antibody reacts with the mu heavy chain of rat IgM. It does not react with other classes of rat immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated HIS40 is used as a detection secondary for rat IgM primary antibodies.