IgM antibody (10R-6568)
Rat monoclonal IgM antibody
Overview
Overview
| Synonyms | Monoclonal IgM antibody, Anti-IgM antibody, IgM antibody, Immunoglobulin M antibody, Ig M antibody, Immunoglobulin M antibody, Ig M antibody, Anti IgM antibody |
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| Specificity | Mouse |
| Applications | ELISA |
| Assay Information | A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate |
Specifications
| Host | Rat |
|---|---|
| Clone | 121-15F9 |
| Isotype | IgG2a kappa |
| Method of Purification | IgM antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated 121-15F9 can be used as a detection secondary for mouse IgM. |
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