IgM antibody (FITC) (61R-1118)

Mouse monoclonal IgM antibody (FITC)

Synonyms Monoclonal IgM antibody, Anti-IgM antibody, Ig M antibody, Ig M antibody, Anti IgM antibody, Immunoglobulin M antibody, IgM antibody, Immunoglobulin M antibody
Specificity Rat
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using IgM antibody (FITC) (61R-1118)

Staining of rat splenocytes with Anti-Rat IgM (FITC) (filled histogram). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Specifications

Host Mouse
Clone HIS40
Isotype IgG1 kappa
Tag/Conjugate FITC
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.5 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The HIS40 monoclonal antibody reacts with the mu heavy chain of rat IgM. It does not react with other classes of rat immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated HIS40 is used as a detection secondary for rat IgM primary antibodies.

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Images

  • Flow Cytometric analysis using IgM antibody (FITC) (61R-1118) | Staining of rat splenocytes with Anti-Rat IgM (FITC) (filled histogram). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Availability: In stock

Price: €216.77
Size: 100 ug
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