IKK beta antibody (70R-50125)

Purified Polyclonal IKK beta antibody

Synonyms Polyclonal IKK beta antibody, Anti-IKK beta antibody, IKBB antibody, TRIP9 antibody, NF-kappa-B inhibitor beta antibody, NF-kappa-BIB antibody, I-kappa-B-beta antibody, IkB-B antibody, IkB-beta antibody, IkappaBbeta antibody, Thyroid receptor-interacting protein 9 antibody, TR-interacting protein 9 antibody, TRIP-9 antibody
Specificity Recognizes endogenous levels of IKK beta protein
Cross Reactivity Mouse, Rat, Bovine
Applications ICC, IF, IHC, WB
Immunogen IKK beta antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human IKK beta as the immunogen

Images

Western blot analysis using IKK beta antibody (70R-50125)

Western blot analysis of IKK beta (pS23) expression in HEK293T LPS-treated (A); SP2/0 LPS-treated (B); PC12 LPS-treated (C); HT29 (D); NIH3T3 TNFa-treated (E) whole cell lysates.

Specifications

Host Rabbit
Method of Purification IKK beta antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance IKK beta is an enzyme that serves as a protein subunit of I?B kinase, which is a component of the cytokine-activated intracellular signaling pathway involved in triggering immune responses.

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Images

  • 

Western blot analysis using IKK beta antibody (70R-50125)



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Western blot analysis of IKK beta (pS23) expression in HEK293T LPS-treated (A); SP2/0 LPS-treated (B); PC12 LPS-treated (C); HT29 (D); NIH3T3 TNFa-treated (E) whole cell lysates.
  • 

Immunohistochemical analysis using IKK beta antibody (70R-50125)



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Immunohistochemical analysis of IKK beta (pS23) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • 

Immunofluorescent analysis using IKK beta antibody (70R-50125)



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Immunofluorescent analysis of IKK beta (pS23) staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €233.95
Size: 100 ul
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