IKK beta antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human IKK beta as the immunogen
Western blot analysis using IKK beta antibody (70R-50125)
Western blot analysis of IKK beta (pS23) expression in HEK293T LPS-treated (A); SP2/0 LPS-treated (B); PC12 LPS-treated (C); HT29 (D); NIH3T3 TNFa-treated (E) whole cell lysates.
IKK beta antibody
Western blot analysis using IKK beta antibody (70R-50125)
Western blot analysis of IKK beta (pS23) expression in HEK293T LPS-treated (A); SP2/0 LPS-treated (B); PC12 LPS-treated (C); HT29 (D); NIH3T3 TNFa-treated (E) whole cell lysates.
Immunohistochemical analysis using IKK beta antibody (70R-50125)
Immunohistochemical analysis of IKK beta (pS23) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis using IKK beta antibody (70R-50125)
Immunofluorescent analysis of IKK beta (pS23) staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
IKK beta antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
IKK beta is an enzyme that serves as a protein subunit of I?B kinase, which is a component of the cytokine-activated intracellular signaling pathway involved in triggering immune responses.