IL-10 protein (His tag) (80-1605)
Recombinant human IL-10 protein (His tag)
Overview
Overview
| Applications | SDS-PAGE |
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Specifications
| Expression System | Baculovirus |
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| Grade & Purity | > 95% pure |
| Method of Purification | SDS-PAGE |
| Molecular Weight | 19.4 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Usage & Assay Information
| Bioactivity | Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 range =5ng/ml. |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | < 1 EU per 1ug of protein (determined by LAL method) |
General Information
| Biological Significance | IL-10, also known as Interleukin-10, is an anti-inflammatory cytokine. It inhibits the synthesis of pro-inflammatory cytokines like IFN-gamma, IL2, IL3, TNF and GM-CSF produced by activated macrophages and byhelper T-cells. This protein has effects in immunoregulation and inflammation and down-regulates theexpression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It acts onmany cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissuedisruption caused by inflammation. This protein controls the inflammatory response of macrophages byreprogramming essential metabolic pathways including mTOR signaling. Recombinant human IL-10, fused to Histagat C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
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| Sequence Information | SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA
ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN
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