IL-10 protein (His tag) (80-1605)

Recombinant human IL-10 protein (His tag)

Specifications

Expression System Baculovirus
Grade & Purity > 95% pure
Method of Purification SDS-PAGE
Molecular Weight 19.4 kDa
Tag/Conjugate His tag
Form & Buffer Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Usage & Assay Information

Bioactivity Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 range =5ng/ml.

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1 EU per 1ug of protein (determined by LAL method)

General Information

Biological Significance IL-10, also known as Interleukin-10, is an anti-inflammatory cytokine. It inhibits the synthesis of pro-inflammatory cytokines like IFN-gamma, IL2, IL3, TNF and GM-CSF produced by activated macrophages and byhelper T-cells. This protein has effects in immunoregulation and inflammation and down-regulates theexpression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It acts onmany cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissuedisruption caused by inflammation. This protein controls the inflammatory response of macrophages byreprogramming essential metabolic pathways including mTOR signaling. Recombinant human IL-10, fused to Histagat C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Sequence Information SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN

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Availability: In stock

Price: €786.91
Size: 100 ug
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