IL12 protein (30R-2169)
Purified recombinant Human IL12 protein
Overview
Overview
| Synonyms | IL 12 protein, p70 protein, IL 12, IL12, IL-12 protein, IL12 protein, Interleukin-12 protein, IL-12 |
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| Specificity | Mouse |
| Species | Human |
| Protein Type | Recombinant |
| Applications | WB |
| Assay Information | It is recommended that the reagent be carefully titrated for optimal /formance in the assay of interest |
Specifications
| Expression System | Insect cells |
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| Grade & Purity | > 98% pure |
| Method of Purification | IL12 protein was purified by affinity chromatography. |
| Molecular Weight | 67 kDa |
| Form & Buffer | Supplied as a sterile liquid with 20 mM NaH2PO4, pH 6.0, 0.2 M NaCl, with 1.0% BSA. 0.22 um filtered. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | WB: < 2 ug/ml |
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| Bioactivity | Measured by dose-dependent induction ofproliferation of PHA-activated human peripheral bloodmononuclear cells. The ED50 is 100 pg/ml, corresponding to a specific activity of 1.0 x 107 Units/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
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| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Interleukin-12 is a heterodimeric 70 kD cytokine composed of two covalently linked, glycosylated chains, 40kD and 35-kD. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides, to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-gamma and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. |
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