IL12 protein (30R-2169)

Purified recombinant Human IL12 protein

Synonyms IL 12 protein, p70 protein, IL 12, IL12, IL-12 protein, IL12 protein, Interleukin-12 protein, IL-12
Specificity Mouse
Species Human
Protein Type Recombinant
Applications WB
Assay Information It is recommended that the reagent be carefully titrated for optimal /formance in the assay of interest

Specifications

Expression System Insect cells
Grade & Purity > 98% pure
Method of Purification IL12 protein was purified by affinity chromatography.
Molecular Weight 67 kDa
Form & Buffer Supplied as a sterile liquid with 20 mM NaH2PO4, pH 6.0, 0.2 M NaCl, with 1.0% BSA. 0.22 um filtered.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations WB: < 2 ug/ml
Bioactivity Measured by dose-dependent induction ofproliferation of PHA-activated human peripheral bloodmononuclear cells. The ED50 is 100 pg/ml, corresponding to a specific activity of 1.0 x 107 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Interleukin-12 is a heterodimeric 70 kD cytokine composed of two covalently linked, glycosylated chains, 40kD and 35-kD. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides, to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-gamma and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

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Availability: In stock

Price: €265.85
Size: 5 ug
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