IL12 protein (His tag) (80R-3978)
Recombinant Human IL12 protein
Overview
Overview
| Synonyms | Recombinant IL12 protein Interleukin 12 (subunit beta/alpha) protein, NKSF1 protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie blue stained SDS-PAGE showing analysis of IL12 protein (His tag) (80R-3978)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 23-328/23-219 aa; * IL-12 is a heterodimer of IL12A and IL12B linked through a disulfide-bond between cysteines in red in below sequences. <br> IL12A(p35) <br>RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH <br> IL12B(p40) <br> IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS |
|---|---|
| Expression System | Insect cells |
| Grade & Purity | > 85% pure |
| Molecular Weight | 34.6 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as liquid in PBS (pH 7.4), 20% glycerol, 1 mM EDTA |
| Concentration | 200 ug/ml |
Storage & Safety
| Storage | Store at 4 deg C for 1-2 weeks. For long term storage, aliquot and store at -20 to -70 deg C. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques. |
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