IL12 protein (Mouse) (30R-2168)

Purified recombinant Mouse IL12 protein

Synonyms IL12, Interleukin-12 protein, IL12 protein, IL-12, IL 12 protein, IL-12 protein, p70 protein, IL 12
Specificity Mouse
Species Mouse
Protein Type Recombinant
Applications FC, IP, WB
Assay Information It is recommended that the reagent be carefully titrated for optimal /formance in the assay of interest

Specifications

Expression System Insect cells
Grade & Purity > 98% pure
Method of Purification IL12 protein was purified by affinity chromatography.
Molecular Weight 65 kDa
Form & Buffer Supplied as a sterile liquid with 10 mM NaH2PO4, pH 6.0, 300mM NaCl, with 1.0% BSA. 0.22 um filtered.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations WB: 1-10 ug/ml
Bioactivity The ED50 of this protein, as measured by IFN gamma induction assay in mouse splenocytes, is less than or equal to 175 pg/ml. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Interleukin-12 is a heterodimeric 70 kD cytokine composed of two covalently linked, glycosylated chains, 40kD and 35-kD. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides, to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.

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