IL12 protein (Mouse) (30R-2251)

Purified recombinant Mouse IL12 protein

Synonyms IL 12, Interleukin-12 protein, IL12, IL-12 protein, IL 12 protein, IL12 protein, IL-12, p70 protein
Specificity Human
Species Mouse
Protein Type Recombinant
Applications FC
Assay Information The pre-titrated test size contains 0.2% BSA.

Specifications

Expression System Insect cells
Grade & Purity > 98% pure
Molecular Weight 57 kDa
Form & Buffer Supplied as a sterile liquid with 10 mM NaH2PO4, pH 6.0, 300mM NaCl. 0.22 um filtered.
Concentration 5 ul (1 ug)/test

Usage & Assay Information

Usage Recommendations FC: 5 ul (1 ug)/test per million cells (100 ul blood)
Bioactivity The ED50 of this protein, as measured by IFN gamma induction assay in mouse splenocytes, is less than or equal to 175 pg/ml. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Interleukin-12 is a heterodimeric 70 kD cytokine composed of two covalently linked, glycosylated chains, 40kD and 35-kD. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides, to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.

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Availability: In stock

Price: €1,631.90
Size: 100 ug
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