IL12 protein (Mouse) (30R-AI007)

Purified recombinant Mouse IL12 protein

Synonyms TCMF protein, CTL maturation factor protein, IL12, TSF protein, IL-12 protein, Interleukin 12 protein, IL-12, IL 12 protein, IL-12 protein, CLMF protein, IL 12, NKSF protein, Cytotoxic lymphocyte maturation factor protein
Species Mouse
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of IL12 protein corresponding to amino acids RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA.
Expression System CHO cells
Grade & Purity > 98% pure
Molecular Weight 75 kDa
Form & Buffer Lyophilized from PBS, pH 7.2, sterile filtered. Reconstitute in PBS to a concentration 0.1 - 1.0 mg/ml.

Usage & Assay Information

Bioactivity Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED50 is = 0.1 ng/ml, corresponding to a specific activity of = 1 x 10^7 units/mg.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance IL12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells.

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