IL15 protein (His tag) (80R-1131)

Purified recombinant Mouse IL15 protein

Synonyms Interleukin 15 protein, IL 15 protein, IL-15 protein, IL-15, Interleukin15 protein, IL 15, IL 15 protein, IL15, MGC9721., IL-15 protein, il15 protein
Species Mouse
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell line: CTLL2 (mouse cytotoxic Tcell) Maintenance Condition: 10% FBS RPMI 1640 with hIL2 Assay Medium: 10% FBS RPMI 1640 without hIL2 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation Time: 24hr (after sample treatment) Concentration Range: 0.039 ng/ml - 40 ng/ml Detection method: MTT assay

Images

Coomassie Blue stained SDS-PAGE of IL15 protein (His tag) (80R-1131)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 49-162 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMNW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 17.6 kDa (152aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in PBS, pH7.4, containing 10% glycerol.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1 ng/ml.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Interleukin (IL)-15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity and has structural similarity to IL-2 that is secreted by mononuclear phagocytes following infection by viruses. Stimulation by IL-15 requires interaction of Il-15 with components of IL-2R, including IL-2Rbeta and probably IL-2R gamma, but not IL-2R alpha. This cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Recombinant human IL-15 was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of IL15 protein (His tag) (80R-1131) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €239.92
Size: 50 ug
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