IL1a antibody (10R-6625)
Armenian Hamster monoclonal IL1a antibody
Overview
Overview
| Synonyms | Monoclonal IL1a antibody, Anti-IL1a antibody, IL 1a, Interleukin-1 alpha antibody, IL-1a antibody, IL 1a antibody, IL-1a antibody, IL1a antibody, IL-1a, IL1a |
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| Specificity | Rat |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
ELISA Assay using IL1a antibody (10R-6625)
Mouse splenocytes (500,000 cells per well) were activated with LPS (1 ug/ml) for 48 hrs in mouse IL1 alpha ELISPOT assay. Control well is medium alone.
Specifications
| Host | Hamster |
|---|---|
| Clone | ALF-161 |
| Isotype | IgG |
| Method of Purification | IL1a antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.25 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The ALF-161 antibody reacts with mouse interleukin-1 alpha. Mouse IL-1 alpha, also called Lymphocyte Activating Factor, Endogenous Pyrogen, Leukocyte Endogenous Mediator, and Mononuclear Cell Factor, is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1 alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1 alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response. |
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