IL1a antibody (10R-6625)

Armenian Hamster monoclonal IL1a antibody

Synonyms Monoclonal IL1a antibody, Anti-IL1a antibody, IL 1a, Interleukin-1 alpha antibody, IL-1a antibody, IL 1a antibody, IL-1a antibody, IL1a antibody, IL-1a, IL1a
Specificity Rat
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

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ELISA Assay using IL1a antibody (10R-6625)

Mouse splenocytes (500,000 cells per well) were activated with LPS (1 ug/ml) for 48 hrs in mouse IL1 alpha ELISPOT assay. Control well is medium alone.

Specifications

Host Hamster
Clone ALF-161
Isotype IgG
Method of Purification IL1a antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.25 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The ALF-161 antibody reacts with mouse interleukin-1 alpha. Mouse IL-1 alpha, also called Lymphocyte Activating Factor, Endogenous Pyrogen, Leukocyte Endogenous Mediator, and Mononuclear Cell Factor, is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1 alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1 alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

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  • ELISA Assay using IL1a antibody (10R-6625) | Mouse splenocytes (500,000 cells per well) were activated with LPS (1 ug/ml) for 48 hrs in mouse IL1 alpha ELISPOT assay. Control well is medium alone.

Availability: In stock

Price: €114.52
Size: 50 ug
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