IL1b antibody (10R-6627)

Mouse monoclonal IL1b antibody

Synonyms Monoclonal IL1b antibody, Anti-IL1b antibody, IL-1b antibody, IL1b, IL 1b, Interleukin-1 beta antibody, IL 1b antibody, IL-1b
Specificity Mouse, Rat
Applications FC, Functional Assay, IP
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Images

Flow Cytometric analysis using IL1b antibody (10R-6627)

Staining of human peripheral blood mononuclear cells stimulated with LPS in the presence of Brefeldin A for 4 hours with CD14 antibody (PE) (left) or CD14 antibody (FITC) (right) followed by intracellularly IL-1 beta antibody (PE).

Specifications

Host Mouse
Clone CRM56
Isotype IgG1
Method of Purification IL1b antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The CRM56 antibody reacts with human and baboon interleukin-1 beta. IL-1 beta is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.

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Images

  • Flow Cytometric analysis using IL1b antibody (10R-6627) | Staining of human peripheral blood mononuclear cells stimulated with LPS in the presence of Brefeldin A for 4 hours with CD14 antibody (PE) (left) or CD14 antibody (FITC) (right) followed by intracellularly IL-1 beta antibody (PE).

Availability: In stock

Price: €106.34
Size: 50 ug
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