IL1b protein (30R-2239)
Purified recombinant Human IL1b protein
Overview
Overview
| Synonyms | Interleukin-1 beta protein, ILb-1, IL1b protein, ILb 1, ILb-1 protein, IL1b, ILb 1 protein |
|---|---|
| Specificity | Mouse |
| Species | Human |
| Protein Type | Recombinant |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Molecular Weight | 18 kDa |
| Form & Buffer | Supplied as a sterile liquid with phosphate buffered saline, pH7.2, 150 mM NaCl. 0.22 um filtered. |
| Concentration | 5 ul (0.06 ug)/test |
Usage & Assay Information
| Usage Recommendations | FC: 5 ul (0.06 ug)/test |
|---|---|
| Bioactivity | The ED50 of this protein, as measured byD10S cell proliferation assay, is less than or equal to 125pg/ml. This corresponds to a specific activity of greater than or equal to 8 x 10e6 Units/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Human IL-1beta, also called lymphocyte activating factor, endogenous pyrogen, leucocyte endogenous mediator, mononuclear cell factor, is produced by a wide variety of cells, including macrophages, dendritic cells, T cells and B cells. IL-1beta is mostly released and has only 20% amino acid homology with IL-1 alpha. The immune regulatory role of IL-1beta is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product