IL1b protein (Mouse) (30R-2153)

Purified recombinant Mouse IL1b protein

Synonyms Interleukin-1 beta protein, ILb-1 protein, ILb 1 protein, IL1b, IL1b protein, ILb 1, ILb-1
Specificity Mouse
Species Mouse
Protein Type Recombinant
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Specifications

Expression System E.coli
Grade & Purity > 98% pure
Method of Purification IL1b protein was purified by affinity chromatography.
Molecular Weight 18 kDa
Form & Buffer Supplied as a sterile liquid with 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, and 1.0% BSA. 0.22 um filtered.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test
Bioactivity Measured by mouse D10S cell proliferation assay. The ED50 is 0.01 ng/ml, corresponding to a specific activity of 1.0 x 10^8 U/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Mouse IL-1beta, also called lymphocyte activating factor, endogenous pyrogen, leucocyte endogenous mediator, mononuclear cell factor, is a ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1beta is mostly cell associated with 23% amino acid homology with IL-1 alpha. The immune regulatory role of IL-1beta is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

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