IL1b protein (Mouse) (30R-2153)
Purified recombinant Mouse IL1b protein
Overview
Overview
| Synonyms | Interleukin-1 beta protein, ILb-1 protein, ILb 1 protein, IL1b, IL1b protein, ILb 1, ILb-1 |
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| Specificity | Mouse |
| Species | Mouse |
| Protein Type | Recombinant |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Method of Purification | IL1b protein was purified by affinity chromatography. |
| Molecular Weight | 18 kDa |
| Form & Buffer | Supplied as a sterile liquid with 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, and 1.0% BSA. 0.22 um filtered. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
|---|---|
| Bioactivity | Measured by mouse D10S cell proliferation assay. The ED50 is 0.01 ng/ml, corresponding to a specific activity of 1.0 x 10^8 U/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Mouse IL-1beta, also called lymphocyte activating factor, endogenous pyrogen, leucocyte endogenous mediator, mononuclear cell factor, is a ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1beta is mostly cell associated with 23% amino acid homology with IL-1 alpha. The immune regulatory role of IL-1beta is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response. |
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