IL2 protein (30R-2157)

Purified recombinant Human IL2 protein

Synonyms Interleukin 2 protein, TCGF protein, IL 2 protein, IL-2 protein, IL 2 protein, IL2, Interleukin-2 protein, IL-2, Aldesleukin protein, T-cell growth factor protein, IL-2 protein, IL 2
Specificity Human
Species Human
Protein Type Recombinant
Applications IHC, IP, WB
Assay Information It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Specifications

Expression System E.coli
Grade & Purity > 98% pure
Method of Purification IL2 protein was purified by affinity chromatography.
Form & Buffer Supplied as a sterile liquid with 40mM Acetate, 0.2M NaCl, pH 5, 1.0% BSA. 0.22 um filtered.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations WB: = 2 ug/ml
Bioactivity The ED50 of this protein, as measured by CTLL-2 proliferation assay, is less than or equal to 175pg/ml. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Human IL-2 is a ~15 kDa factor produced mainly by activated CD4 T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.

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