IL2 protein (His tag) (80R-1105)

Purified recombinant Mouse IL2 protein

Synonyms IL-2 protein, IL2, Aldesleukin protein, IL-2 protein, IL 2 protein, Interleukin-2 protein, IL-2, Interleukin 2 protein, T-cell growth factor protein, IL 2 protein, IL 2, TCGF protein
Species Mouse
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell line: CTLL2 (mouse cytotoxic Tcell) Maintenance Condition: 10% FBS RPMI 1640 with hIL2 Assay Medium - 10% FBS RPMI 1640 without hIL2 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation Time: 24hr (after sample treatment) Concentration Range: 0.039 ng/ml - 40 ng/ml Detection method: MTT assay

Images

Coomassie Blue stained SDS-PAGE of IL2 protein (His tag) (80R-1105)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 21-169 amino acids: MGSSHHHHHH SSGLVPRGSH MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSVISTSPQ
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 19.5 kDa (170 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in PBS, pH7.4, containing 20% glycerol, and 0.1M NaCl.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is < 2.5 ng/ml. Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 2.5 ng/ml.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Interleukin-2 (IL-2), also known as T cell growth factor (TCGF), is an immunoregulatory lymphokine produced by T-cells in response to proteinic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant mouse IL-2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of IL2 protein (His tag) (80R-1105) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €169.35
Size: 100 ug
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