IL27 protein (30R-2179)

Purified recombinant Human IL27 protein

Synonyms p29 protein, IL-27 protein, IL-27, EBI3 protein, IL 27 protein, IL 27, IL-30 protein, IL27, Interleukin-27 protein, IL27 protein
Specificity Mouse
Species Human
Protein Type Recombinant
Applications ELISA
Assay Information A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/ml - 4 pg/ml should be included in each ELISA plate

Specifications

Expression System 293 cells
Grade & Purity > 98% pure
Method of Purification IL27 protein was purified by affinity chromatography.
Molecular Weight 55 kDa
Form & Buffer Supplied as a sterile liquid with 20 mM NaH2PO4, pH 6.0, 0.6MNaCl, with 1.0% BSA. 0.22 um filtered.
Concentration 500 ug/ml

Usage & Assay Information

Bioactivity Measured in a bioassay of IL-27-mediated inhibition of IL-2 production by mouse splenocytes activated with immobilized anti-CD3 and soluble anti-CD28 antibodies. The ED50 for this is typically 50 ng/ml, corresponding to a specific activity of 2.0 x 10^4

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 non-covalently linked to an IL-12p35-related protein, p28. IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naïve CD4+ T cells mRNA expression analysis of IL-27 receptor components suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-gamma-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rbeta2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naïve CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.

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Availability: In stock

Price: €429.45
Size: 10 ug
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