IL27 protein (Mouse) (30R-2259)

Purified recombinant Mouse IL27 protein

Synonyms IL-30 protein, IL 27 protein, Interleukin-27 protein, IL27, p29 protein, IL-27, IL 27, IL27 protein, EBI3 protein, IL-27 protein
Specificity Human
Species Mouse
Protein Type Recombinant
Applications FC
Assay Information A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Specifications

Expression System 293 cells
Grade & Purity > 98% pure
Molecular Weight 55 kDa
Form & Buffer Supplied as a sterile liquid with 10 mM sodium phosphate, pH 7.2,0.22 um filtered.
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.03 ug/test
Bioactivity Measured by induction of IL-6 production by human peripheral blood mononuclear cells. The ED50 for this effect is typically 250 - 500 ng/ml.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 non-covalently linked to an IL-12p35-related protein, p28. IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naïve CD4+ T cells mRNA expression analysis of IL-27 receptor components suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-gamma-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rbeta2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naïve CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.

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Availability: In stock

Price: €2,368.10
Size: 100 ug
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