IL3 protein (30R-2159)
Purified recombinant Human IL3 protein
Overview
Overview
| Synonyms | Interleukin-3 protein, P-cell-stimulating factor protein, IL 3 protein, Mast cell growth factor protein, IL 3, MCGF protein, IL3, IL-3, IL3 protein, IL-3 protein, Multipotential colony-stimulating factor protein, Hematopoietic growth factor protein |
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| Specificity | Mouse |
| Species | Human |
| Protein Type | Recombinant |
| Applications | FC, IP |
| Assay Information | It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
Functional Assay using IL3 protein (30R-2159)
Proliferation of TF-1 cells in response to Human IL3 protein
Specifications
| Expression System | E.coli |
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| Grade & Purity | > 95% pure |
| Method of Purification | IL3 protein was purified by affinity chromatography. |
| Form & Buffer | Supplied as a sterile liquid with 120 mM phosphate Buffer, 0.15MNaCl, pH 7.0, 1% BSA, 0.22 um filtered. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Bioactivity | The ED50 measured in a TF-1 proliferation assay is typically less than 0.15 ng/ml, corresponding to a specific activity of greater than 7x10^6 Units/mg. |
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Storage & Safety
| Storage | Store at -70 deg C or less. |
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| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Interleukin 3, also called Mast Cell Growth Factor, Multi-Colony Stimulating Factor, Eosinophil-CSF, is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. Human IL-3 has no activity on mouse cells. |
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