IL3 protein (30R-2243)

Purified recombinant Human IL3 protein

Synonyms P-cell-stimulating factor protein, Multipotential colony-stimulating factor protein, IL 3, MCGF protein, IL-3 protein, Interleukin-3 protein, Mast cell growth factor protein, IL 3 protein, IL-3, IL3 protein, IL3, Hematopoietic growth factor protein
Specificity Mouse
Species Human
Protein Type Recombinant
Applications FC
Assay Information A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test.

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Functional Assay using IL3 protein (30R-2243)

Proliferation of the TF1 cell line in response to Human IL3 protein

Specifications

Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 15 kDa
Form & Buffer Supplied as a sterile liquid with 20mM phosphate nuffer, 0.15MNaCl, pH 7.0, 0.22 um filtered.
Concentration 200 ug/mL

Usage & Assay Information

Usage Recommendations FC: 5 ul (0.125 ug)/test
Bioactivity The ED50 measured in a TF-1 proliferation assay is typically less than 0.15 ng/ml, corresponding to a specific activity of greater than 7x10^6 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Interleukin 3, also called Mast Cell Growth Factor, Multi-Colony Stimulating Factor, Eosinophil-CSF, is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. Human IL-3 has no activity on mouse cells.

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  • Functional Assay using IL3 protein (30R-2243) | Proliferation of the TF1 cell line in response to Human IL3 protein

Availability: In stock

Price: €793.46
Size: 100 ug
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