IL3 protein (Mouse) (30R-2158)
Purified recombinant Mouse IL3 protein
Overview
Overview
| Synonyms | IL-3, MCGF protein, Multipotential colony-stimulating factor protein, IL3 protein, IL3, Hematopoietic growth factor protein, IL-3 protein, Interleukin-3 protein, IL 3 protein, IL 3, Mast cell growth factor protein, P-cell-stimulating factor protein |
|---|---|
| Specificity | Mouse |
| Species | Mouse |
| Protein Type | Recombinant |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Method of Purification | IL3 protein was purified by affinity chromatography. |
| Molecular Weight | 14 kDa |
| Form & Buffer | Supplied as a sterile liquid with phosphate buffered saline, pH 7.2, 150 mM NaCl, 1.0% BSA. 0.22 um filtered. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
|---|---|
| Bioactivity | Measured by mouse MC/9 cell proliferation assay. The ED50 is 0.1 ng/ml, corresponding to a specific activity of 1.0 x 10^7 Units/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Mouse IL-3, also called Mast Cell Growth Factor, Multi-Colony Stimulating Factor, Eosinophil-CSF, is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. |
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