IL33 protein (Mouse) (30R-2270)

Purified recombinant Mouse IL33 protein

Synonyms Interleukin-33 protein, IL33 protein, IL-33, IL 33 protein, IL33, IL 33, IL-33 protein
Specificity Mouse
Species Mouse
Protein Type Recombinant
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Specifications

Expression System E.coli
Grade & Purity > 97% pure
Molecular Weight 15 kDa
Form & Buffer Supplied as a sterile liquid with 10 mM phosphate, 150 mMNaCl, 2 mM DTT, pH 7.2. 0.22 um filtered.
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.125 ug/test
Bioactivity Measured by D10.G4.1 cell proliferation assay.The ED50 is 0.03 ng/ml, corresponding to a specific activity of 3.0 x 107 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Mouse IL-33, also called NF-HEV and DVS 27, is a 30 kDa proinflammatory cytokine produced by endothelial and epithelial cells. IL-33 is released during necrotic cell death but was first found in the nucleus of endothelial cells. This dual pattern of expression is reminiscent of alarmins also known as endogeneous danger signals. Other known alarmins are IL-1 alpha and HMGB1. IL-33 has been identified as the ligand for ST2. ST2 is stably expressed on mast cells and T2 effector T cells and is functionally associated with T2-mediated inflammation. Although IL-33 was initially reported to be processed by Caspase-1, recent data indicate that Caspase-3 or -7 are capable of processing IL-33 at more physiologic concentrations. IL-33 plays an immune regulatory role by inducing IL-5 and IL-13 in vitro and in vivo and activating basophils, eosinophils and mast cells. In addition to its role in proimflammation, it may also decrease inflammation through interactions with IL-1 thereby blocking its effect. Mouse IL-33 share 55% amino acid sequence identity with human and 90% with rat.

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Availability: In stock

Price: €1,798.77
Size: 100 ug
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