IL33 protein (Mouse) (30R-2270)
Purified recombinant Mouse IL33 protein
Overview
Overview
| Synonyms | Interleukin-33 protein, IL33 protein, IL-33, IL 33 protein, IL33, IL 33, IL-33 protein |
|---|---|
| Specificity | Mouse |
| Species | Mouse |
| Protein Type | Recombinant |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 97% pure |
| Molecular Weight | 15 kDa |
| Form & Buffer | Supplied as a sterile liquid with 10 mM phosphate, 150 mMNaCl, 2 mM DTT, pH 7.2. 0.22 um filtered. |
| Concentration | 200 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.125 ug/test |
|---|---|
| Bioactivity | Measured by D10.G4.1 cell proliferation assay.The ED50 is 0.03 ng/ml, corresponding to a specific activity of 3.0 x 107 Units/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Mouse IL-33, also called NF-HEV and DVS 27, is a 30 kDa proinflammatory cytokine produced by endothelial and epithelial cells. IL-33 is released during necrotic cell death but was first found in the nucleus of endothelial cells. This dual pattern of expression is reminiscent of alarmins also known as endogeneous danger signals. Other known alarmins are IL-1 alpha and HMGB1. IL-33 has been identified as the ligand for ST2. ST2 is stably expressed on mast cells and T2 effector T cells and is functionally associated with T2-mediated inflammation. Although IL-33 was initially reported to be processed by Caspase-1, recent data indicate that Caspase-3 or -7 are capable of processing IL-33 at more physiologic concentrations. IL-33 plays an immune regulatory role by inducing IL-5 and IL-13 in vitro and in vivo and activating basophils, eosinophils and mast cells. In addition to its role in proimflammation, it may also decrease inflammation through interactions with IL-1 thereby blocking its effect. Mouse IL-33 share 55% amino acid sequence identity with human and 90% with rat. |
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