IL4 antibody (10R-6628)
Rat monoclonal IL4 antibody
Overview
Overview
| Synonyms | Monoclonal IL4 antibody, Anti-IL4 antibody, IL 4 antibody, Lymphocyte stimulatory factor 1 antibody, IGG1 induction factor antibody, B-cell stimulatory factor 1 antibody, IL4 antibody, IL-4, IL-4 antibody, IL4, IL-4 antibody, B-cell IgG differentiation factor antibody, IL 4, BSF-1 antibody, Interleukin-4 antibody, B-cell growth factor 1 antibody |
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| Specificity | Human |
| Applications | FC, Functional Assay |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
ELISA Assay using IL4 antibody (10R-6628)
Splenocytes from 8 week old mice pre-immunized with OVA/IFA were recall-activated with OVA antigen for 36 hours in Mouse IL4 ELISPOT assay. Control well is medium alone.
Specifications
| Host | Rat |
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| Clone | 11B11 |
| Isotype | IgG1 kappa |
| Method of Purification | IL4 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.5 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 11B11 antibody reacts with mouse interleukin-4, a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23. |
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