IL4 antibody (10R-6628)

Rat monoclonal IL4 antibody

Synonyms Monoclonal IL4 antibody, Anti-IL4 antibody, IL 4 antibody, Lymphocyte stimulatory factor 1 antibody, IGG1 induction factor antibody, B-cell stimulatory factor 1 antibody, IL4 antibody, IL-4, IL-4 antibody, IL4, IL-4 antibody, B-cell IgG differentiation factor antibody, IL 4, BSF-1 antibody, Interleukin-4 antibody, B-cell growth factor 1 antibody
Specificity Human
Applications FC, Functional Assay
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

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ELISA Assay using IL4 antibody (10R-6628)

Splenocytes from 8 week old mice pre-immunized with OVA/IFA were recall-activated with OVA antigen for 36 hours in Mouse IL4 ELISPOT assay. Control well is medium alone.

Specifications

Host Rat
Clone 11B11
Isotype IgG1 kappa
Method of Purification IL4 antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.5 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The 11B11 antibody reacts with mouse interleukin-4, a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

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Images

  • ELISA Assay using IL4 antibody (10R-6628) | Splenocytes from 8 week old mice pre-immunized with OVA/IFA were recall-activated with OVA antigen for 36 hours in Mouse IL4 ELISPOT assay. Control well is medium alone.

Availability: In stock

Price: €102.25
Size: 50 ug
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