IL4 protein (30R-2245)

Purified recombinant Human IL4 protein

Synonyms IL-4, IL 4 protein, Interleukin-4 protein, IL4, IL 4, IL-4 protein, IL4 protein
Specificity Mouse
Species Human
Protein Type Recombinant
Applications FC
Assay Information APC Rat IgM Isotype Control has been tested by flow cytometric analysis of mouse splenocytes and should be used at the same concentration as the experimental antibody.

Specifications

Expression System E.coli
Grade & Purity > 98% pure
Molecular Weight 15 kDa
Form & Buffer Supplied as a sterile liquid with phosphate buffered saline, pH7.2, 150 mM NaCl. 0.22 um filtered.
Concentration 200 ug/ml

Usage & Assay Information

Bioactivity The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 175 pg/ml.This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Human IL-4 is a ~15-19 kDa factor also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23.

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Availability: In stock

Price: €1,189.37
Size: 100 ug
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