IL4 protein (Mouse) (30R-2244)

Purified recombinant Mouse IL4 protein

Synonyms IL4, Interleukin-4 protein, IL-4 protein, IL 4, IL 4 protein, IL4 protein, IL-4
Species Mouse
Protein Type Recombinant
Applications FC
Assay Information A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Specifications

Expression System E.coli
Grade & Purity > 98% pure
Molecular Weight 13 kDa
Form & Buffer Supplied as a sterile liquid with 20mM phosphate, 0.38M NaCl,pH 6.5. 0.22 um filtered.
Concentration 200 ug/mL

Usage & Assay Information

Usage Recommendations FC: 5 ul (0.125 ug)/test
Bioactivity The ED50 of this protein, as measured by CTLL-2 proliferation assay, is less than or equal to 350 pg/ml. This corresponds to a specific activity of greater than or equal to 2.9 x 10e6 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

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Availability: In stock

Price: €1,419.22
Size: 100 ug
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