IL4 protein (Mouse) (30R-2244)
Purified recombinant Mouse IL4 protein
Overview
Overview
| Synonyms | IL4, Interleukin-4 protein, IL-4 protein, IL 4, IL 4 protein, IL4 protein, IL-4 |
|---|---|
| Species | Mouse |
| Protein Type | Recombinant |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Molecular Weight | 13 kDa |
| Form & Buffer | Supplied as a sterile liquid with 20mM phosphate, 0.38M NaCl,pH 6.5. 0.22 um filtered. |
| Concentration | 200 ug/mL |
Usage & Assay Information
| Usage Recommendations | FC: 5 ul (0.125 ug)/test |
|---|---|
| Bioactivity | The ED50 of this protein, as measured by CTLL-2 proliferation assay, is less than or equal to 350 pg/ml. This corresponds to a specific activity of greater than or equal to 2.9 x 10e6 Units/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23. |
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