IL6 protein (Mouse) (30R-2163)

Purified recombinant Mouse IL6 protein

Synonyms IL6 protein, Interleukin-6 protein, IL 6 protein, IL 6, IL-6, IL-6 protein, IL6
Specificity Mouse
Species Mouse
Protein Type Recombinant
Applications FC, IHC, IP, WB
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Specifications

Expression System E.coli
Grade & Purity > 98% pure
Method of Purification IL6 protein was purified by affinity chromatography.
Molecular Weight 19 kDa
Form & Buffer Supplied as a sterile liquid with 40% acetonitrile, 0.1% trifluoroacetic acid, 0.5% BSA. 0.22 um filtered.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.5 ug/test
Bioactivity The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 5 pg/ml.This corresponds to a specific activity of greater than or equal to 2 x 10e8 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Mouse IL-6 is a ~21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

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