IL7 protein (30R-2249)
Purified recombinant Human IL7 protein
Overview
Overview
| Synonyms | IL7, Interleukin-7 protein, IL 7, IL-7, IL-7 protein, IL 7 protein, IL7 protein |
|---|---|
| Specificity | Mouse |
| Species | Human |
| Protein Type | Recombinant |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Molecular Weight | 18 kDa |
| Form & Buffer | Supplied as a sterile liquid with 20mM Tris, 50mM NaCl, pH 7.8.0.22 um filtered. |
| Concentration | 200 ug/mL |
Usage & Assay Information
| Usage Recommendations | FC: 5 ul (0.5 ug)/test |
|---|---|
| Bioactivity | Measured by dose-dependent proliferation of PHA activated human PBMCs. The ED50 is typically 2.0 ng/ml, corresponding to a specific activity of approximately 5x10^5Units/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Human IL-7, also called lymphopoietin 1, a pre-B cell growth factor, is a ~28 kDa factor produced by bone marrow and thymic stromal cell and spleen cells. IL-7 is a stromal cell-derived growth factor for progenitor B cells and T cells. The main population in the thymus responsive to IL-7 is CD4-CD8-. It also stimulates proliferation and differentiation of mature T cells and NK cells. |
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