IL7 protein (30R-2249)

Purified recombinant Human IL7 protein

Synonyms IL7, Interleukin-7 protein, IL 7, IL-7, IL-7 protein, IL 7 protein, IL7 protein
Specificity Mouse
Species Human
Protein Type Recombinant
Applications FC
Assay Information A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test.

Specifications

Expression System E.coli
Grade & Purity > 98% pure
Molecular Weight 18 kDa
Form & Buffer Supplied as a sterile liquid with 20mM Tris, 50mM NaCl, pH 7.8.0.22 um filtered.
Concentration 200 ug/mL

Usage & Assay Information

Usage Recommendations FC: 5 ul (0.5 ug)/test
Bioactivity Measured by dose-dependent proliferation of PHA activated human PBMCs. The ED50 is typically 2.0 ng/ml, corresponding to a specific activity of approximately 5x10^5Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Human IL-7, also called lymphopoietin 1, a pre-B cell growth factor, is a ~28 kDa factor produced by bone marrow and thymic stromal cell and spleen cells. IL-7 is a stromal cell-derived growth factor for progenitor B cells and T cells. The main population in the thymus responsive to IL-7 is CD4-CD8-. It also stimulates proliferation and differentiation of mature T cells and NK cells.

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Availability: In stock

Price: €1,388.96
Size: 100 ug
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