Influenza A IgM ELISA Kit (55R-RE56521)

ELISA kit for detection of Influenza A IgM in the research laboratory

Synonyms Influenza A ELISA Kit
Applications ELISA
Assay Information The Influenza A IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Influenza A antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-touse standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Influenza A antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use antihuman-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgM antibodies is directly proportional to the intensity of the color. NOTE that samples containing hyper-immune levels of anti-human IgG antibodies may cause false positive results in this assay. Any patient samples testing positive should be pre-diluted with IgG adsorbent and re-tested to confirm elevated levels of IgM.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C.
Biohazard Information All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as pote

General Information

Biological Significance The influenza infection is an acute feverish virus infection, which principally leads to an illness of the respiratory tract and appears as an epidemic or pandemic. The Influenza viruses form a virus group with principally similar morphological, chemical and biological features. The types A, B and C have been defined. The determination of the Influenza type (A, B, and C) gives both the clinician and epidemiologist important indications for further actions. Thus during an Influenza A epidemic, the epidemiological importance and derived measures for the protection of the individual and population primarily stand in the foreground together with the severity of the clinical symptoms.

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Influenza A IGM Elisa Kit Protocol

Added: Aug 25, 2015


Availability: In stock

Price: €154.78
Size: 96 wells
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