Immunofluorescent staining using Inhibin beta A antibody (70R-13185)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Inhibin beta-A antibody at 1:200 dilution.
Inhibin beta A antibody
Immunofluorescent staining using Inhibin beta A antibody (70R-13185)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Inhibin beta-A antibody at 1:200 dilution.
Immunohistochemical staining using Inhibin beta A antibody (70R-13185)
Immunohistochemical staining of paraffin-embedded CLEAR CELL OVCA xenograft using Inhibin beta A antibody at a dilution of 1:500
Western blot analysis using Inhibin beta A antibody (70R-13185)
Western blot analysis of 30 ug whole cell lysate (A:H1299; B:HeLa S3; C:Hep G2) using a 10 % SDS PAGE gel and Inhibin beta A antibody at a dilution of 1:1000
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
Inhibin beta A antibody was purified by antigen-affinity chromatography
Molecular Weight
47 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.