Flow Cytometric assay using IRF3 antibody (10R-1207)
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-IRF3 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
IRF3 antibody
Flow Cytometric assay using IRF3 antibody (10R-1207)
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-IRF3 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
Western Blot analysis of immunized recombinant protein using IRF3 antibody (10R-1207)
Left: IRF3 staining. Right: Coomassie Blue staining of immunized recombinant protein.
Immunofluorescent staining using IRF3 antibody (10R-1207)
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-IRF3 antibody.
Specifications
Host
Mouse
Clone
IRF35I218
Isotype
IgG1
Method of Purification
IRF3 antibody was purified using protein G column chromatography from culture supernatant of hybridomacultured in a medium containing bovine IgG-depleted(approximately 95%) fetal bovine serum.
Form & Buffer
IRF3 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information
This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv
General Information
Biological Significance
IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferoninduced genes.