IRF5 antibody (10R-1026)

Mouse monoclonal IRF5 antibody

Synonyms Monoclonal IRF5 antibody, Anti-IRF5 antibody, interferon regulatory factor 5 antibody, irf5 antibody, IRF 5 antibody, IRF-5 antibody, IRF-5, IRF5, IRF 5
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, IHC, IP, WB
Immunogen IRF5 antibody was raised in mouse using recombinant human IRF-5 (176-240aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of IRF5 antibody (10R-1026)

The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 10T1
Isotype IgG1 kappa
Source Anti-human IRF-5 antibody, clone 10T1, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IRF-5 protein.
Method of Purification IRF5 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:1,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Interferon regulatory factor 5 (IRF-5) is a member of the IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs (IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.

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Images

  • Western Blot analysis of IRF5 antibody (10R-1026) | The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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