ISG15 protein (Calmodulin tag) (80R-1055)
Purified recombinant Human ISG15 protein (Calmodulin tag)
Overview
Overview
| Synonyms | ISG 15 protein, Ubiquitin cross reactive protein precursor., 15 kDa ubiquitin-like modifier protein, Interferon induced 15 kDa protein protein, GIP2 protein, IFI15 protein, ISG15 ubiquitin like modifier protein, Interferon alpha inducible protein protein, UCRP protein, ISG15 protein, ISG15, Interferon induced 17 kDa/15 kDa protein protein, ISG 15 protein, 15 kDa ubiquitin-like modifier IFI 15 protein, ISG-15, Interferon-stimulated gene protein, ISG 15, Interferon induced 17 kDa protein protein, Interferon Stimulated Gene 15 protein, Interferon-stimulated gene protein, Interferon stimulated protein 15 kDa protein, ISG-15 protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of ISG15 protein (Calmodulin tag) (80R-1055)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-157 amino acids: MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMGWDLTVKM LAGNEFQVSL SSSMSVSELK AQITQKIGVH AFQQRLAVHP SGVALQDRVP LASQGLGPGS TVLLVVDKCD EPLNILVRNN KGRSSTYEVR LTQTVAHLKQ QVSGLEGVQD DLFWLTFEGK PLEDQLPLGE YGLKPLSTVF MNLRLRGG |
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| Expression System | E.coli |
| Grade & Purity | > 98% pure |
| Molecular Weight | 34 kDa (308aa), confirmed by MALDI-TOF. |
| Tag/Conjugate | Calmodulin tag |
| Form & Buffer | Supplied as a liquid in in 20mM Tris pH 8.0, 0.1M NaCl, 1mM DTT |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | The interferon-stimulated gene, 15 kDa (ISG15) is an interferon regulated gene that is induced as a primary response to diverse microbial and cell stress stimuli, and encodes the founding member of the ubiquitin-like protein family. ISG15 shares several common properties with other ubiquitin-like molecules (UBLs), but its activity is tightly regulated by specific signaling pathways that have a role in innate immunity. Recombinant human ISG15 was expressed in E. coli and purified by conventional chromatography techniques. |
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