JAml antibody (10R-6532)

Armenian Hamster monoclonal JAml antibody

Synonyms Monoclonal JAml antibody, Anti-JAml antibody, JAml antibody, Anti JAml antibody, AMICA antibody, AMICA antibody, Junctional Adhesion Molecule-Like antibody, Junctional Adhesion Molecule-Like antibody
Specificity Mouse
Applications FC, Functional Assay, IP, WB
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Images

Flow Cytometric analysis using JAml antibody (10R-6532)

Staining of mouse JAML-transfected CHO cells with 0.5 ug of Armenian Hamster IgG Isotype Control (open histogram) or 0.5 ug of Anti-Mouse JAML Purified (filled histogram) followed by Anti-Armenian Hamster IgG (FITC). Total viable cells were used for analysis.

Specifications

Host Hamster
Clone 4E10
Isotype IgG
Method of Purification JAml antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The 4E10 monoclonal antibody reacts with mouse JAml, also known as AMICA. JAml is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAml is expressed on polymorphonuclear leukocytes, and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAml and epithelial coxsackie and adenovirus receptor. JAml is also expressed on lymphocyte subsets, such as gamma delta T cell lines, and can provide co-stimulatory signals for cell activation.

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Images

  • Flow Cytometric analysis using JAml antibody (10R-6532) | Staining of mouse JAML-transfected CHO cells with 0.5 ug of Armenian Hamster IgG Isotype Control (open histogram) or 0.5 ug of Anti-Mouse JAML Purified (filled histogram) followed by Anti-Armenian Hamster IgG (FITC). Total viable cells were used for analysis.

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Price From: Price: €134.97

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Price From: Price: €134.97
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